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iκb superrepressor  (ATCC)


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    Structured Review

    ATCC iκb superrepressor
    Iκb Superrepressor, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i%CE%BAb+superrepressor/JB6+Cl+30-7b/10__1091_slash_mbc__e12___10___0737-258-5-29
    Average 96 stars, based on 993 article reviews
    iκb superrepressor - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Expressing:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Generated:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Produced:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Cotransfection:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Transfection:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Plasmid Preparation:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).

    Selection:

    Article Title: Cells surviving fractional killing by TRAIL exhibit transient but sustainable resistance and inflammatory phenotypes
    Article Snippet: HCT116 cells were obtained from the American Type Culture Collection (Manassas, VA); single-cell clones were generated by serial dilution and were cultured as described (Aldridge et al., 2011). .. MCF10A cells stably expressing the IκB superrepressor (Boehm et al., 2007) were generated using a retrovirus produced by cotransfection (FuGENE 6 Transfection Reagent; Roche, Indianapolis, IN) of 293T cells (American Type Culture Collection) with pCL-ampho and pBabe-Puro-IκBalpha-mut (plasmid 15291; Addgene, Cambridge, MA) and selection with puromycin. .. Singlecell clones generated by serial dilution were tested for complete (<0.1%) lack of nuclear translocation of NF-κB after stimulation with TNFa (PeproTech, Rocky Hill, NJ).



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